The Alexandria District of the Louisiana Conference of the United Methodist Church originated through the 1971 merger of Louisiana’s two racially segregated conferences. From 1968 to 1971, Louisiana’s Conference A (for white churches) and Conference B (for black churches) existed as a temporary measure to work towards racial integration of the Louisiana Conference following the denomination’s 1968 dissolution of the Central Jurisdiction (a racially segregated unit for black Methodists).

In 1972, the district reported 9,535 members. The district consisted of congregations in central Louisiana stretching from Olla (north) to Rosedale (south) and Sieper (west) to Vidalia (east).

In 2013, the Alexandria District of the Louisiana Conference of the United Methodist Church was dissolved. The congregations that constituted the district were assigned to other districts: Acadiana District of the Louisiana Conference of the United Methodist Church, Lake Charles District of the Louisiana Conference of the United Methodist Church, Monroe District of the Louisiana Conference of the United Methodist Church, and Shreveport District of the Louisiana Conference of the United Methodist Church.